Operator: ScotRail
Calling Points:
| 20:45 | Prestwick Town | On time |
| 20:48 | Newton-on-Ayr | On time |
| 20:52 | Ayr | On time |
| 20:45 | Prestwick Town | On time |
| 20:48 | Newton-on-Ayr | On time |
| 20:52 | Ayr | On time |
| 20:49 | Troon | On time |
| 20:52 | Barassie | On time |
| 20:56 | Irvine | On time |
| 21:00 | Kilwinning | On time |
| 21:14 | Johnstone | On time |
| 21:18 | Paisley Gilmour Street | On time |
| 21:30 | Glasgow Central | On time |
| 21:16 | Troon | On time |
| 21:19 | Barassie | On time |
| 21:23 | Irvine | On time |
| 21:27 | Kilwinning | On time |
| 21:41 | Johnstone | On time |
| 21:45 | Paisley Gilmour Street | On time |
| 21:56 | Glasgow Central | On time |
| 21:15 | Prestwick Town | On time |
| 21:19 | Newton-on-Ayr | On time |
| 21:22 | Ayr | On time |
| 21:46 | Troon | On time |
| 21:48 | Barassie | On time |
| 21:53 | Irvine | On time |
| 21:57 | Kilwinning | On time |
| 22:11 | Johnstone | On time |
| 22:15 | Paisley Gilmour Street | On time |
| 22:26 | Glasgow Central | On time |
| 21:46 | Prestwick Town | On time |
| 21:50 | Newton-on-Ayr | On time |
| 21:54 | Ayr | On time |
| 22:16 | Troon | On time |
| 22:18 | Barassie | On time |
| 22:23 | Irvine | On time |
| 22:27 | Kilwinning | On time |
| 22:41 | Johnstone | On time |
| 22:45 | Paisley Gilmour Street | On time |
| 22:59 | Glasgow Central | On time |
| 22:15 | Prestwick Town | On time |
| 22:18 | Newton-on-Ayr | On time |
| 22:23 | Ayr | On time |