Operator: ScotRail
Previous Calling Points:
| 05:15 | Ayr | Unknown |
| 05:17 | Newton-on-Ayr | Unknown |
| 05:20 | Prestwick Town | Unknown |
| 05:22 | Prestwick Intl Airport | Unknown |
| 05:15 | Ayr | Unknown |
| 05:17 | Newton-on-Ayr | Unknown |
| 05:20 | Prestwick Town | Unknown |
| 05:22 | Prestwick Intl Airport | Unknown |
| 05:36 | Ayr | Unknown |
| 05:38 | Newton-on-Ayr | Unknown |
| 05:41 | Prestwick Town | Unknown |
| 05:43 | Prestwick Intl Airport | Unknown |